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one of several worries in the event or generation of SMC from ASCs is always to enhance microenvironments for effective linear-precise differentiation, cell proliferation, and maintenance of contractile phenotype. Oxygen is a vital ingredient of the optimal microenvironment [9]. Although most cell cultures and expansions are carried out in vitro at twenty% oxygen focus, the actual oxygen tensions of numerous tissues in physiological surroundings tend to be lower: oxygen concentration in adipose tissue is somewhere around two–eight% [10].
To examine the physiological effect of hypoxia on differentiation of ASC, a collagen gel contraction assay was utilized, which measures the adjust in gel measurement with time.
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